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. 2016 Oct 5;14(5):4622–4628. doi: 10.3892/mmr.2016.5808

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — PCDHGA3 gene knockdown. (A) PCDHGA3 mRNA was detected in the FL-derived cell lines, FL18 and FL318. Knockdown of the PCDHGA3 gene was confirmed by (B) reverse transcription-quantitative polymerase chain reaction, (C) western blotting and (D) immunofluorescence. The siRNA, s31924, was selected for further analysis because it yielded the greatest PCDHGA3 mRNA inhibition ratio (55.9%) relative to untreated FL18 cells, original magnification ×100. (E) Cell proliferation was significantly suppressed in PCDHGA3 siRNA-treated FL18 cells at 72 h. (F) Coexpression of PCDHGA3 and TNFRSF6B was observed in the FL18 cell line by double immunofluorescence staining, original magnification ×200. *P<0.01; **P<0.001. PCDHG, protocadherin γ; FL, follicular lymphoma; siRNA, small interfering RNA; TNFRSF6B, tumor necrosis factor receptor superfamily member 6B; DAPI, 4,6-diamidino-2-phenylindole.