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. 2016 Nov 8;2:16040. doi: 10.1038/celldisc.2016.40

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Copyright © 2016 The Author(s)

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An exposed hydrophobic residue-containing segment destabilizes unassembled Ubl4A. (a) A schematic illustration of the Ubl4A constructs used in the study. (b) The C-terminus of Ubl4A contains a ‘degron’. HEK293T cells transfected with the indicated plasmids were treated with dimethyl sulfoxide (−) or 10 μm MG132 (+) for 20 h. Whole-cell extracts were analyzed by immunoblotting. (c) Structural analysis of the Ubl4A-Bag6 interactions. Panel 1, The C-terminus of Ubl4A contains 3 helices (H1, H2 and H3). Panel 2, A surface view of the Ubl4A C-terminus. Residues are colored by hydrophobicity. Panel 3, Bag6 shields the hydrophobic residues in H1 and H2. Panel 4 shows a close-up view of the boxed area in panel 2. (d) Cycloheximide chase analysis of cells transfected with GFP appended with either the H1 or H2 segment from Ubl4A.