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. 2016 Oct 12;14(5):4643–4649. doi: 10.3892/mmr.2016.5838

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — (A) Liquid chromatography-mass spectrometry detection of emodin eluted from Sirt1-loaded affinity columns. The α peak (in red curve) and β peak (in green curve) indicate the detected samples from the control and emodin-bound Sirt1-loaded columns, respectively. The α peak represents the specific peak for emodin. (B) Immunoblots of Sirt1 further confirmed the direct binding of emodin to Sirt1. ‘-’ indicates non-emodin-loaded affinity columns, ‘+’ indicates emodin-loaded affinity columns. The samples comprised lung tissue lysate and Sirt1 protein. mAbs, monoclonal antibodies; Sirt1, surtuin 1.