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. 2016 Oct 12;14(5):4680–4686. doi: 10.3892/mmr.2016.5826

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Copyright: © Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — CBD-VEGF promoted hepatocyte proliferation and suppressed hepatocyte apoptosis and HSC activation. Immunohistochemical staining of Ki67 and α-SMA in addition to a TUNEL assay were performed in the liver sections of each group. (A) Upper panels, Ki67 immunohistochemical staining (magnification, ×400); middle panels, TUNEL assay for evaluation of hepatocyte apoptosis (magnification, ×400); lower panels, α-SMA immunohistochemical staining (magnification, ×100). (B) Statistical analysis of proliferative hepatocytes, (C) TUNEL-positive hepatocytes and (D) α-SMA positive area of each group. n=5 for each group. Data are presented as the mean ± standard deviation; **P<0.01, *P<0.05. CBD-VEGF, collagen-binding domain-vascular endothelial growth factor; HSC, hepatic stellate cells; α-SMA, α-smooth muscle actin; NS, normal saline.