Figure 2.

ZOL increases autophagic activity instead of inhibition of lysosomal degradation. Human umbilical vein endothelial cells were pretreated with vehicle or 1 µM CQ for 1 h, then exposed to 100 µM ZOL for 48 h. The protein levels of (A) LC3B-I and -II, and (B) SQSTM1 were determined by western blot and densitometric analysis was performed. Data are presented as the mean ± standard error, n=6. **P<0.01 vs. V alone; ^##P<0.01 vs. ZOL + V group; ZOL, Zoledronate; V, vehicle; CQ, chloroquine; LC3B, microtubule-associated proteins 1A/1B light chain 3B; SQSTM1, sequestome 1.