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GST pull-down assay of WWP2 and PTEN. (A) GST-WWP2 or GST (1 μM) and n-PTEN or 4p-PTEN (1 μM) were incubated with glutathione agarose resin overnight. The pull-down of PTEN by WWP2 was analyzed by Western blotting using the anti-PTEN antibody. (B) Quantification of 4p-PTEN relative to n-PTEN pull-down by WWP2, average over replicates (n = 3) with the standard error shown.
