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. 2016 Sep 6;30(12):4083–4097. doi: 10.1096/fj.201600430R

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This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Mobility of ER chaperone BiP is retarded in Z-α₁-antitrypsin-expressing cells. CHO cells coexpressing BiP-mCherry and either YFP-M-α₁-antitrypsin (A) or YFP-Z-α₁-antitrypsin (B) were subjected to 2-color FRAP. Retardation of recovery of BiP-mCherry fluorescence (red squares) was noted in YFP-Z-α₁-antitrypsin vs. YFP-M-α₁-antitrypsin-expressing cells (P < 0.0001). C) Two-color FRAP was performed with region of interest sufficiently small to lie within one large inclusion. Area of bleached YFP-Z-α₁-antitrypsin persists for more than 2 min. BiP-mCherry fluorescence was completely bleached within inclusion but began to recover during 5 min, while adjacent inclusions partially dimmed. Scale bars, 10 µm. D) Quantification of BiP-mCherry fluorescence intensity in bleached vs. adjacent inclusion 1 min after bleaching relative to immediate postbleaching intensity.