Figure 4.

A) To evaluate the direct effect of IGFBP-3 (IBP3) on palmitate (PA)-induced IL-8 expression, IGFBP-3 was overexpressed in the presence of palmitate (0.5 mM) in Huh-7 cells. B–D) Overexpression of IGFBP-3 suppressed IKK2 protein and both JNK and NF-κB activation in palmitate-treated Huh-7 cells. E, F) This was accompanied by decreased IL-8 mRNA expression (E) and IL-8 protein secretion (F) into the medium. Ctrl, control. Means ± sd from 3 independent experiments are shown for all graphical data.