The expression of Gal3 and NLRP3 inflammasome components are induced in patients with PBC. A) Liver tissues from healthy subjects (Ctrl) and patients with PBC were processed for RT-qPCR to evaluate the expression of Gal3, NLRP3, ASC, and IL-1β. A) The mRNA levels of Gal3, NLRP3, the adaptor ASC and the downstream effector IL-1β were all significantly elevated in livers from patients with PBC. *P < 0.05; means ± sem; n = 4. B) Western blot and densitometry analyses showed that the protein levels of Gal3 and NLRP3 were increased in PBC livers. *P < 0.05; means ± sem; n = 4. C) Immunohistochemistry revealed enhanced signals for Gal3 (a, c) and NLRP3 (b, d) in the livers from patients with PBC compared with normal livers (NL) from healthy control subjects.