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. 2016 Sep 6;30(12):4149–4158. doi: 10.1096/fj.201600569R

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This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Higher-resolution X-ray fluorescence images. A) Top: Br-SA. Bottom: a surface plot generated based on the red area in the top images. B) Top: Br-PA. Bottom: a surface plot generated based on the red area in the top images. The yellow-framed area was measured at a higher resolution. Red arrows indicate the direction presented in the surface plots; white arrows indicate the spot-like Br distribution. BrKα, a bromine X-ray emission line; Br-SA, cells treated with Br-labeled stearic acid; Br-PA, cells treated with Br-labeled palmitic acid; DIC, differential interference contrast image; Zn, zinc. A brighter color indicates higher signal intensity. Color bar, femtograms per sqare micrometer; scale bar, 10 μm.