Figure 1.

Insulin-stimulated nitric oxide (NO^•) production and endothelial nitric oxide synthase (eNOS) phosphorylation in human aortic endothelial cells incubated in 5 mM glucose, 25 mM glucose, and 25 mM glucose + 3 µM γ-CEHC. (a) NO^• production after insulin stimulation. (b, top): Total and insulin-stimulated phosphorylation of eNOS at serine 1177 (p-eNOS ^Ser1177). (b, bottom): Ratio of eNOS phosphorylation at Ser473 to total eNOS expression. All values are presented as means ± SE; n  = 4–6 independent experiments. *P < 0.05 vs. 5 mM glucose