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. Author manuscript; available in PMC: 2016 Nov 9.
Published in final edited form as: Nature. 2015 Jan 19;520(7545):99–103. doi: 10.1038/nature14027

Figure 3. Viability, colonization and immunostimulatory potential of in vitro-grown SFB.

Figure 3

a, SFB length after growth on TC7s on TWs; b, Quantitation and c, Gram stain of SFB growth on TC7s on TWs before and after a 3-day sub-culturing of the 5-μm filtrate; d-k, Analysis of germfree C57BL/6 mice gavaged with either Fil/IOs mix, IOs, or feces of SFB-monoassociated mice (SFBVivo); d, Quantification of ileum-associated SFB; e/k, Host gene expression in the ileal lamina propria; Representative flow cytometry plots and quantitation of B220+B-cell (f/g) and CD45+CD3+CD4+T-cell (i/j) frequencies of the indicated markers; h, fecal secretory IgA quantitation by ELISA.