Skip to main content
. 2016 Nov 9;11(11):e0166073. doi: 10.1371/journal.pone.0166073

Fig 5. Viability of human ASCs spheroids formed into lockyballs.

Fig 5

(A, B) DAPI staining of human ASCs spheroid formed in the absence (A) and into (B) lockyballs (blue: DAPI staining, green: autofluorescent lockyballs) shows cell nuclei without any detectable morphological signs of apoptosis or cell death. Bar size: 50 micrometers. (C) Viable cells identified by 7AAD exclusion (dead cells in R1) using flow cytometry for quantitative analysis. Dot-plot graph is representative of digested mass from 90 spheroids in the absence and into lockyballs. Twenty thousand events were acquired in each tube. (D) Percentage of viable cells in spheroids in the absence (blue bar; 97,6±1,1) and into (red bar; 97,3±1,2) lockyballs. Graph represents the mean ± standard error of the mean of three independent experiments (p › 0,99). Fold expression for SOX9 (E) and for RUNX2 (F) master genes in spheroids in the absence and into lockyballs. (E, F) Graphs represent the mean ± standard error of the triplicates from two independent cell samples. Students´ t test was used and resulted a p = 0,1965 (sample 1) and p = 0,6444 (sample 2) for SOX9; p = 0,7620 (sample 1) and p = 0,1953 (sample 2) for RUNX2.