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. 2016 Nov 9;11(11):e0166187. doi: 10.1371/journal.pone.0166187

Fig 1. Mouse rPrP is a more efficient substrate for amplification of CWD seeding activity.

Fig 1

Serial dilutions of CWD-positive (a) or negative (b) elk brain homogenates were used as seeds for RT-QuIC reactions. As a substrate, either mouse (left panel) or deer rPrP (right panel) were used. Y-axes indicate the relative ThT fluorescence units, x-axes show the reaction time. A threshold to determine positive reactions was calculated by using the average baseline fluorescence plus 5 standard deviations, which is indicated as a solid line at app. 50,000 RFU. (c) The time to reach the threshold (y-axis) was determined for each individual reaction. Dilutions of 2x10-2 to 2x10-5 of reactions with either mouse or deer rPrP were included, and the averages of the time to reach the threshold are shown. Bars represent standard deviations. For each dilution mouse and deer rPrP were compared and the difference between the two groups was statistically evaluated using unpaired student’s t-test (GraphPad Prism software; ** = p-value < 0.01; ns = not significant).