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. 2016 Nov 9;11(11):e0166187. doi: 10.1371/journal.pone.0166187

Fig 3. CWD seeding activity is detectable in fecal samples of elk at the pre-clinical stage of disease.

Fig 3

(a) Fecal homogenates (10% w/v) of elk orally infected with CWD prions and taken after different time points post infection (dpi) were subjected to NaPTA precipitation and 10fold concentration. These samples were either used undiluted or in two serial dilutions as indicated to seed RT-QuIC reactions with mouse rPrP as a substrate. Substrate replacement was done after a reaction time of 25 hours. The Prnp genotype is indicated, with MM animals being homozygous for methionine at amino acid 132, LM indicates heterozygosity for methionine/leucine. (b) The time to reach the threshold (lag time) was determined for each positive sample and dilutions of 2x10-2 to 2x10-5 of the CWD-positive elk brain homogenate. Averages of 4 replicates are shown, bars represent the standard deviation. Statistical analysis was done using ANOVA and post-hoc analysis by Dunnett’s Multiple Comparison Test (ns: p-value > 0.05; GraphPad Prism software).