Fig 1. Disruption of NAC subunits rescues toxicity associated with the [PSI+] prion.
(A) The nascent-polypeptide associated complex consists of the proteins Egd1 (β), Btt1 (β’), and Egd2 (α) that share homology within their NAC domains. (B) Sup35 under control of the copper-inducible promoter CUP1 was induced in [psi-] WT, [PSI+] WT, and [PSI+] NAC deletion strains by growth on selective media containing 350μM CuSO4. The empty vector (EV) control contained the pCUP1 vector. Single horizontal white line separates non-contiguous spots from the same plates. Dashed black line separates spots from different plate of identical media. The “egd1Δt” designation indicates the strain isolated from the transposon screen.