Table 1. Primer sequences used for the present genome amplification.
| Primer name | Sequence (5′→3′) | Polarity | Regiona |
|---|---|---|---|
| Sydney2012-1F | GTGAATGAAGATGGCGTCTAAC | + | 1–22 |
| Sydney2012-1R | GGTAAATCCTAGCACCAAACCT | - | 1045–1066 |
| Sydney2012-2F | TGATTGGACCTTCGCAGGCATAG | + | 871–893 |
| Sydney2012-2R | TCTAGCCTCTCATGGAGTAACC | - | 2055–2076 |
| Sydney2012-3F | CATCCATGATGCCCTCAGGT | + | 1660–1679 |
| Sydney2012-3R | GATTTGCTTGATAGGGACTCCG | - | 3148–3169 |
| Sydney2012-4F | GCAACCGAAGAGGACTTCTGTGAAG | + | 2813–2837 |
| Sydney2012-4R | TGAGGAGCCAGTGGGCGATGGAAT | - | 4497–4520 |
| 290 H | GATTACTCCAGGTGGGACTCCAC | + | 4295–4317 |
| 290 I | GATTACTCCAGGTGGGACTCAAC | ||
| 290 J | GATTACTCCAGGTGGGATTCAAC | ||
| 290 K | GATTACTCCAGGTGGGATTCCAC | ||
| G2SKR | CCACCTGCATAACCATTGTACAT | - | 5367–5389 |
| COG2F | CARGARBCNATGTTYAGRTGGATGAG | + | 5003–5029 |
| VN3T20 | GAGTGACCGCGGCCGCT20 | - | Poly A |
a Each sequence number of primer sets region is listed for Sydney 2012 strain (GenBank accession No. JX459908).