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. 2015 Sep 23;56(10):6144–6161. doi: 10.1167/iovs.15-16569

Figure 2.

Figure 2

Changes in expression of genes encoding extracellular matrix proteins in response to ET-1 treatment in RGCs. Expression of mmp2, mmp3, timp1, tnc, and aspn was detected by microarray and real-time PCR. There was no change detected in mRNA level of mmp2 in RGCs with ET-1 treatment from microarray and real-time PCR; however, a significant upregulation of mmp3 expression was detected in both assays (8.2-fold, n = 3 and 5.4-fold, n = 12, respectively) with ET-1 treatment. An appreciable 2.2-fold increase (P = 0.065, t-test, n = 3) of timp1 (an endogenous inhibitor of MMPs) was found by microarray, and a 2.4-fold increase (P < 0.001, t-test, n = 12) of timp1 was found in real-time PCR in RGCs treated with ET-1. The mRNA level of tnc in RGCs in response to ET-1 treatment was significantly upregulated to 7.5- and 17.0-fold in microarray and real-time PCR, respectively. There was a 2.4- and 5.3-fold decrease of mRNA of asporin (aspn) detected from microarray and real-time PCR after 24-hour treatment of ET-1 in RGCs. (mRNA levels [mean ± SEM] are represented in bar graph; in microarray, the vehicle-treated cells served as control; in real-time PCR, results were normalized to the expression of cyclophilin A, and values were compared to vehicle-treated control cells). Statistical analysis was performed using t-test (*P < 0.05, **P ≤ 0.01, #P ≤ 0.001).