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. 2015 Sep 23;56(10):6144–6161. doi: 10.1167/iovs.15-16569

Figure 9.

Figure 9

Endothelin-1 mediated changes in expression of members of the Bcl-2 and caspase gene family. Endothelin-1 treatment of RGCs induced a significant decrease in the mRNA levels of bcl-2, bak1, and bid (P < 0.01, t-test, n = 3–6) determined by real-time PCR (A). A downregulation of mRNA expression of casp2 and casp7 was found by real-time PCR in RGCs treated with ET-1, which was statistically significant (B). Expression of capn6 was downregulated in ET-1–treated RGCs determined by microarray analysis, and the same trend also was detected in real-time PCR. Messenger RNA level of capn6 in ET-1 treatment in real-time was 12.9% of control (P < 0.001, t-test, n = 9 [B]). *P < 0.05, **P < 0.01, #P < 0.001; mRNA levels (mean ± SEM) are represented in bar graph; in microarray, the vehicle control served as control; in real-time PCR, results were normalized to the expression of cyclophilin A, and compared to vehicle-treated control cells.