DS-CARS images at (a) 1260 cm−1, (b) 1300 cm−1, (e) 1450 cm−1 and (f) 1650 cm−1 of a mixture of fish and olive oil droplets. Image 50 × 50 pixels, pixel time 50 ms. The droplets marked with arrows are difficult to identify in (a), (b), (e) and (f). (c) and (g) Intensity ratio image of 1260 cm−1 over 1300 cm−1 (I1260/I1300) and 1450 cm−1 over 1650 cm−1 (I1450/I1650). Fish and olive oil droplets appear blue and reddish yellow, respectively. (d) and (h) DS-CARS spectra of the oil droplets marked as A, B, C and D in parts (a), (b), (e) and (f), respectively. All the traces in (d) and (h) are vertically displaced for clarity.