RBC signal and plasma volume measurements are used to determine a hematocrit index. False color volume and hematocrit maps are overlaid on grayscale structural OCT images, with large arteries and veins marked A and V, respectively. A) Log scale red blood cell (RBC) content as measured by the qualitative baseline OCT angiogram. B) A quantitative map of plasma volume per vessel shown in log scale. C) Qualitative hematocrit index for individual vessels. D) Mean hematocrit index (red line) and individual hematocrit index measurements (blue lines) across the microvascular layers of four rats. IR – Total Inner Retina, ONF/GCL – Optic Nerve Fiber/Ganglion Cell Layer, IPL – Inner Plexiform Layer, OPL – Outer Plexiform Layer, and CC – Choriocapillaris.