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. 2016 Sep 14;7(10):4021–4033. doi: 10.1364/BOE.7.004021

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Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.

Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.

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Fig. 1 — Schematic of the light-sheet microscope objectives, oriented 45° to the vertical, and sample slide. Primed coordinates: tissue reference frame. Unprimed coordinates: microscope reference frame. y = y′, while x and z are tilted 45° relative to x′ and z′ such that the focal plane of the illumination objective (left) is parallel to x − y, and z is parallel to the optical axis of the detection objective.