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FWHM lateral measurements of fluorescent resolution markers in 0.1M PBS and 47% TDE/PBS buffers. Sample size indicates number of beads detected by spot finding algorithm.
| Beam Type | Buffer Medium | x–FWHM (μm) | y–FWHM (μm) | Sample Size |
|---|---|---|---|---|
|
| ||||
| Gaussian | PBS | 0.83 ± 0.05 | 0.77 ± 0.04 | 50 |
| TDE | 1.1 ± 0.1 | 1.0 ± 0.1 | 129 | |
|
| ||||
| Airy | PBS | 0.79 ± 0.02 | 0.83 ± 0.06 | 48 |
| TDE | 1.1 ± 0.2 | 1.0 ± 0.1 | 124 | |
