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Linear fits to the plot of FWHM vs depth from Figure 6. FWHM at surface (y-intercept) and FWHM/depth (gradient). Sample size (number of detected beads) was 86 for GLSM, 257 for ALSM.
| Beam Type | Fit Orientation | FWHM at surface (μm) | FWHM/Depth (μm/ μm) |
|---|---|---|---|
|
| |||
| Airy | Lateral | 1.18 ± 0.04 | (1.7 ± 0.2) × 10−3 |
| Axial | 1.44 ± 0.06 | (2.6 ± 0.3) × 10−3 | |
|
| |||
| Gaussian | Lateral | 1.1 ± 0.1 | (1.9 ± 0.6) × 10−3 |
| Axial | 1.16 ± 0.09 | (2.2 ± 0.6) × 10−3 | |
