Fig. 11.

Changing plane of focus provides independent imaging of the three vascular stratifications of the mouse retina. Split detector intensity images (left) and corresponding motion contrast images (right). Despite lack of a confocal pinhole, stratifications are independently imaged. Vessels parallel to the knife edge are most visible in the split–detector images (red arrow), whereas contrast from orthogonal vessels are less visible (white arrow). Motion contrast images show a double line profile in vessels parallel to the knife edge (red arrowhead) while a single line can be observed in orthogonal vessels (white arrowhead).