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. 2015 Oct 1;84(1):41–55. doi: 10.1111/tpj.12957

Figure 2.

Figure 2

CYCD7;1 expression in the central cell of the female gametophyte overcomes cell‐cycle arrest.

(a–c) Digital interference contrast (DIC) micrographs of unfertilized ovules. Wild‐type (WT) ovules show a single nucleus (orange) in central cell (blue) (a), whereas several nuclei are visible in independent lines of endosperm‐targeted CYCD7;1 expression (end CYCD7;1; two representative examples are shown in b and c). Scale bar: 20 μm.

(d) Bar chart showing the number of nuclei in the central cell of ovules sampled from WT (top panel) and end CYCD7;1 (bottom panel) emasculated pistils.

(e) Estimation of expression level of end CYCD7;1 transcripts in four independent transgenic lines (end CYCD7;1 A–D) by semi‐quantitative RT‐PCR.

(f) Number of nuclei in the seed endosperm 24 h after pollination (n = 51).

(g) Fertilization event triggers the synthesis of proanthocyanidins (PAs) in the maternal integuments marking the acquisition of seed coat fate. PA‐accumulating cells are decorated red after vanillin staining. No PA was detected by this method in end CYCD7;1 ovules. Scale bars: 40 μm.