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In Escherichia coli, the synthetic Chlamydomonas reinhardtii chloroplast trn W UCA gene does not permit full length CrCD protein expression from the crCD** gene. (a) Western analysis using equalized lysates of E. coli DH5α carrying four different plasmids. The blot was probed with an αHA antibody to detect HA‐tagged CrCD protein. (b) Growth curve of E. coli DH5α carrying the four different plasmids.
