Immunohistochemical detection of viral antigen in 4-week-old chickens intranasally inoculated with106 EID50 of poultry H5N2 viruses. A/turkey/Minnesota/12582/2015 (A, B, D, G, I), and A/chicken/Iowa/13388/2015 (C, E, F, H). A. Viral antigen (in red) in epithelium of air capillaries and mononuclear cells in the lung. B. Viral antigen in cardiac myocytes. C. Viral antigen in acinar cells in pancreas. D. Viral antigen in mononuclear cells in the spleen. E. Viral staining in adrenal corticotropic cells. F. Viral antigen in neurons and ependymal cells in the brain. G. Viral staining in histiocytes in the thymus. H. Viral staining in tubular epithelial cells in kidney. I. Viral staining in vascular endothelial cells and infiltrating mononuclear cells in eyelid. Magnification 40X.