Table 6.

Non-Synonymous substitutions found in the poultry H5N2 HPAI isolates when compared to A/Northern pintail/Washington/40964/2014 (H5N2) (A/Np/WA/14).

Protein	Codon Number	Amino Acid Change	Nucleotide Position	Nucleotide Change	Codon Change	A/Tk/AR/15	A/Tk/MN/15	A/Tk/SD/15	A/Ck/IA/15
HA	7	L -> P	20	T -> C	CTT -> CCT				X
HA	8	L -> F	22	C -> T	CTT -> TTT	X	X	X	X
HA	82	M -> I	246	G -> A	ATG -> ATA				X
HA	130	N -> T	389	A -> C	AAT -> ACT	X	X	X	X
HA	157	S -> P	469	T -> C	TCC -> CCC	X	X	X	X
HA	338	L -> Q	1,013	T -> A	CTA -> CAA				X
NA	150	H -> N	448	C -> A	CAT -> AAT	X
NA	253	R -> K	758	G -> A	AGA -> AAA	X	X	X	X
NA	368	E -> K	1,102	G -> A	GAA -> AAA	X	X	X	X
NA	412	V -> A	1,235	T -> C	GTT -> GCT	X	X	X
NA	416	S -> G	1,246	A -> G	AGC -> GGC				X
PB2	386	L -> V	1,156	T -> G	TTA -> GTA	X	X	X	X
PB2	649	V -> I	1,945	G -> A	GTA -> ATA	X	X	X	X
PB1-F2	15	H -> P	44	A -> C	CAC -> CCC		X
PB1	180	E -> D	540	A -> C	GAA -> GAC				X
PB1	215	R -> K	644	G -> A	AGG -> AAG		X	X	X
PB1	317	M -> V	949	A -> G	ATG -> GTG				X
PB1	531	K -> R	1,592	A -> G	AAG -> AGG	X
PB1	667	I -> T	2,000	T -> C	ATC -> ACC			X
PB1	723	R -> L	2,168	G -> T	CGA -> CTA		X
PA	337	A -> V	1,010	C -> T	GCT -> GTT		X	X	X
PA	475	A -> T	1,423	G -> A	GCA -> ACA				X
NP	109	I -> T	326	T -> C	ATC -> ACC				X
NP	347	I -> L	1,039	A -> C	ATC -> CTC	X
M2	78	Q -> R	233	A -> G	CAG -> CGG	X	X	X	X
NS1	64	I -> K	191	T -> A	ATA -> AAA	X
NS1	176	I -> T	527	T -> C	ATT -> ACT	X	X	X	X
NS1	203	W -> R	607	T -> C	TGG -> CGG	X
NS1	217	K -> T	650	A -> C	AAA -> ACA		X	X	X
NEP	60	N -> H	178	A -> C	AAC ->		X	X	X