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. Author manuscript; available in PMC: 2017 Dec 1.

Published in final edited form as: Virology. 2016 Sep 12;499:9–22. doi: 10.1016/j.virol.2016.08.031

Fig. 2 — Assays were performed as in Figure 1, specifically for the co-cultures (with contact). In addition: (A) M-MDSCs (upper well) were physically separated from responder cells (lower well) using a transwell system (see Materials and Methods). (B) A Griess’ assay was performed with stimulated B-cells (lower well) co-cultured with M-MDSCs (upper well) in the absence of cell contact. Data shown are from representative experiments, with a similar pattern of results (as shown for both A and B) observed in at least 5 experiments each. Significance levels: *, p<0.05; **, p<0.01; NS, not significant.

Fig. 2 — Assays were performed as in Figure 1, specifically for the co-cultures (with contact). In addition: (A) M-MDSCs (upper well) were physically separated from responder cells (lower well) using a transwell system (see Materials and Methods). (B) A Griess’ assay was performed with stimulated B-cells (lower well) co-cultured with M-MDSCs (upper well) in the absence of cell contact. Data shown are from representative experiments, with a similar pattern of results (as shown for both A and B) observed in at least 5 experiments each. Significance levels: *, p<0.05; **, p<0.01; NS, not significant.