Table 3.
DNA damage according to the comet assay for the assessment of the genotoxicity of polyphenolic extract from Q. sideroxyla bark.
| Treatment | Comet class | ||||
|---|---|---|---|---|---|
| Total | 0 | 1 | 2 | 3 | |
| Blood sample (4 h) | |||||
| Negative control | 8 ± 4.8 | 92.5 ± 4.1 | 7 ± 3.5 | 0.5 ± 0.8 | 0 ± 0 |
| CPA (50 mg/kg) | 67.3 ± 6.9a | 66 ± 3.1a | 11.8 ± 3.3a | 11 ± 2.4a | 11.1 ± 1.0a |
| CE100 | 16.8 ± 6.3ab | 85.8 ± 4.9b | 12.1 ± 3.5a | 1.33 ± 0.8b | 0.66 ± 0.8 |
| CE200 | 39.5 ± 6.2abc | 69.8 ± 5.1a | 22.5 ± 6.0ab | 6 ± 2.09abc | 1.6 ± 0.8b |
| Blood sample (24 h) | |||||
| Negative control | 18.5 ± 6.9 | 88.6 ± 3.1 | 7.6 ± 3.3 | 3.1 ± 2.4 | 1.5 ± 1.04 |
| CPA (50 mg/kg) | 140.3 ± 21.2a | 37.5 ± 5.5a | 11 ± 6.2a | 25.2 ± 8.4a | 26.3 ± 6.3a |
| CE100 | 21.3 ± 6.1b | 85.5 ± 4.0b | 9.1 ± 0.7a | 3.3 ± 1.6b | 1.8 ± 1.4b |
| CE200 | 60 ± 14.3ab | 55.3 ± 5.9ab | 33.3 ± 3.4ab | 7.3 ± 4.0b | 4 ± 2.6b |
Each value represents the mean of n = 6 observations ± standard deviation. a: significant difference versus negative control group, b: versus CPA 50 mg/kg, and c: versus CE100; (p ≤ 0.05) LSD, comparing the treatments separately at 4 and 24 hours.
CPA: group treated with cyclophosphamide; CE100: group treated with crude extract at dose of 100 mg/kg; CE200: group treated with crude extract at dose of 200 mg/kg.