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. 2016 Nov 10;7:1678. doi: 10.3389/fpls.2016.01678

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Copyright © 2016 Guo, Chen, Hussain, Zhang and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histochemical analysis of GUS activity in transgenic Arabidopsis plants. (A–J) GUS staining at different developmental stages; (K), GUS activity responding to ABA, drought, NaCl, and cold treatments. (A) 2-day-old seedlings, (B) 3-day-old seedling, (C) 6-day-old seedling, (D) rosette leaf, (E,F) flowers, (G) young siliques, (H) mature siliques, (I) Col-0, and (J) 2-week-old seedlings. (K) 4-week-old seedlings supplemented with 100 μM ABA, 20% PEG6000, and 100 mM NaCl. For cold treatment, the seedlings were transferred to a 4°C growth chamber in the dark. GUS activity was assayed after 12 h treatment. The values are expressed as means ± SD (n = 4). *Indicates statistically significant difference at (p <0.05) and **indicates a highly statistically significant difference at (p <0.01) compared with CK. Bar = 5 mm. The line with arrow shows the magnified tissue portion.