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. 2016 Nov 10;7:1811. doi: 10.3389/fmicb.2016.01811

FIGURE 4.

FIGURE 4

KinC/KinD and YwcC-mediated pathways regulate γ-PGA production in B. subtilis.(A) Shown is the colony mucoidy phenotype on LB agar media by various mutants in 3610. Strains used in this assay include 3610 (WT), RL4573 (ΔkinAΔkinB), RL4262 (ΔkinC), RL4569 (ΔkinD), RL5273 (ΔkinCΔkinD), YY214 (Δspo0A), YY233 (Δspo0A ΔepsH), YY208 (Δspo0A ΔabrB), YC668 (ΔabrB), and YY201 (ΔabrB ΔepsH). Cells were streaked out on LB plates and incubated for 12 h at 37°C before pictures were taken. Both the streaking plates and enlarged colonies were shown. (B) Shown in (B) are the same as in (A) except that different mutants of 3610 were applied. Strains used here include 3610 (WT), YC295 (ΔywcC), YC299 (ΔywcC ΔepsH), RL3852 (ΔepsH), YY198 (ΔywcCΔepsHΔpgs), YY199 (ΔywcCΔepsHΔslrR), and YY200 (ΔywcCΔepsHΔslrR Δpgs), and RL4637 (ΔdegSU). (C) The colony mucoidy phenotype by a ΔslrR derivative (FC100) expressing slrR from an IPTG-inducible hyperspank promoter at the amyE locus. Scale bars in the panels showing the streaking plate and the enlarged colonies represent 5 and 1 mm in length, respectively. This applies to similar panels in this figure.