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. 2016 Nov 10;7:1811. doi: 10.3389/fmicb.2016.01811

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Copyright © 2016 Yu, Yan, Chen, Jin, Guo and Chai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The influence of γ-PGA production on colony biofilm robustness is medium-dependent. (A) The colony biofilm phenotype on MSgg from the wild type strains and the pgs mutants in both 3610 and SM21. Scale bar: 5 mm in length. (B) SDS-PAGE for methylene blue stained samples containing purified γ-PGA from YY170 (Δpgs in SM21, used as a negative control), 3610, and SM21, prepared from cells growing in either LBGM or MSgg. (C) Assays of β-galactosidase activities from a SM21 derivative (YY245) containing the promoter-lacZ transcriptional reporter for the pgs operon (P_SM21pgsB-lacZ) at the amyE locus show different expression of the reporter in LBGM and MSgg. Cells were grown in shaking conditions in LBGM (unfilled squares) or MSgg (filled diamonds) broth over a period of 7 h. Assays were done in triplicate. Error bars represent standard deviations.