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. 2016 Nov 10;7:1686. doi: 10.3389/fpls.2016.01686

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Copyright © 2016 Iurlaro, De Caroli, Sabella, De Pascali, Rampino, De Bellis, Perrotta, Dalessandro, Piro, Fry and Lenucci.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of a 42°C pre-treatment, applied to enzyme extracts in vitro, on their residual XET activity (subsequently assayed at 25°C). The extracts had been prepared from () root apical regions, () root sub-apical regions, and () shoots of non-stressed 3-day-old wheat seedlings. Three cultivars were compared: (A) Ardente, (B) Creso, and (C) Svevo, which differ in heat tolerance. XET activity, remaining in the extract after the in vitro heat pre-treatment, is given as the fluorescence intensity of the generated xyloglucan–XXXGol-SR expressed as a percentage of that produced by the corresponding untreated extracts. Data are the mean ± standard deviation of six independent replicates (n = 6).

Inline graphic — Effect of a 42°C pre-treatment, applied to enzyme extracts in vitro, on their residual XET activity (subsequently assayed at 25°C). The extracts had been prepared from () root apical regions, () root sub-apical regions, and () shoots of non-stressed 3-day-old wheat seedlings. Three cultivars were compared: (A) Ardente, (B) Creso, and (C) Svevo, which differ in heat tolerance. XET activity, remaining in the extract after the in vitro heat pre-treatment, is given as the fluorescence intensity of the generated xyloglucan–XXXGol-SR expressed as a percentage of that produced by the corresponding untreated extracts. Data are the mean ± standard deviation of six independent replicates (n = 6).