Fig. 2.

Steps from embryo transformation to shifting of plants in soil pots a Germination of Embryos in sterilized flasks, b Embryos isolation from seeds, c Agrobacterium-treated embryos are co-cultivated on MS medium, d Agrobacterium-treated embryos growing on MS medium (4 days), e Inoculation of embryos in test tubes containing MS media with kanamycin selection, f Roots developing in MS rooting medium, g Transgenic plants being shifted in soil pots, h putative transgenic plants in soil pots covered with polythene bags, i Acclimatization of putative transgenic plants