Table 1.
Internal primer details for gene amplification of RNAi gene construct and real-time PCR
| Name | Primer sequence | Length | Tm | Product size (bp) |
|---|---|---|---|---|
| C2-int (L) | TCATAATCTAAACCAAACAGGGAAA | 25 | 60.11 | 540 |
| C2-int (R) | TTACAATCAGGTCCTTCAGCAAA | 23 | 60.99 | |
| ClCuV Burewala (F) | CGAAAGAAGAAGGAGAAAAA | 23 | 53.0 | 203 |
| ClCuV Burewala (R) | AGCAAGAGGAGGACAGCAGA | 20 | 59.4 | |
| ClCuV Multan beta (F) | GTTCCGCTGGTTGTCATTTC | 20 | 55.4 | 268 |
| ClCuV Multan beta (R) | CCTCTTCAGTTCCGTTTTTC | 21 | 54.6 |
Bold values are the important facts and figures about results