Skip to main content
. 2016 Nov 1;111(9):1954–1964. doi: 10.1016/j.bpj.2016.09.017

Figure 4.

Figure 4

The E34A mutation in Cpx promotes Syb unraveling. (A) The mutation accelerates unraveling of the bundle under external forces (the blue dotted line represents the average for the MD replicas of the native SNARE-Cpx complex; Fig. 1B). (B) Subsequent time points along the trajectory of the mutant complex under external forces suggest the following scenario for Syb unraveling: 1) the partially unraveled Syb C-terminus contacts Cpx (60 ns); 2) Cpx transitions from a closed to an open state, separating Syb from the bundle (80 ns); Cpx transitions back to the closed state, with Cpx AH moving along the partially unraveled Syb C-terminus (90 ns); and 4) Cpx transitions into the open state, further unraveling Syb (270 ns). Two perpendicular views are shown at each time point.