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. 2016 Oct 3;111(9):1827–1830. doi: 10.1016/j.bpj.2016.09.022

Figure 2.

Figure 2

Properties of adhered filopodia inferred from TIRF images. (A) Illustration of TIRF illumination for a semiinfinite region of a cell versus the circular cross section of a filopod. (B) Histogram of filopod radii determined from images of the membrane marker, GPI-tdTomato. (C) With R determined from b, the volume marker (TFP) images were used to estimate the void fraction εf for each filopod. (D) With R determined from b and assuming εf = 0.62 for each filopod, the predicted value of Φ for the volume marker (Eq. 5) is compared to the measured value. To see this figure in color, go online.