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. 2016 Oct 3;111(9):1827–1830. doi: 10.1016/j.bpj.2016.09.022

Figure 3.

Figure 3

Quantifying PI3K signaling in filopodia. (A) Histogram of Tf/dKD estimated from Eq. 7. (B) Adjusted fluorescence intensity of a filopod (arrow). Scale bar = 10 μm. (C) Pseudocolor TIRF image showing the specification of a region adjacent to a filopod. Scale bar = 10 μm. (D) The adjusted fluorescence of each filopod, 1+Tf/dKD, is compared to that of the adjacent region, 1+Ta/dKD. (E) Spatiotemporal map showing structures identified as lamellipodia (white) and filopodia (colored according to Tf/dKD). (Right) The bar graph shows the mean adjusted intensities of filopodia associated with front (n = 310), stationary (n = 547), and rear (n = 479) regions (see also Fig. S2). The error bars are 95% confidence intervals. To see this figure in color, go online.