Table 2.
Temperature-dependent Kinetic Parameters for the Interactions between the nSH3 Domain and PRM758
| Temperature (K) | kon (108 M−1 s−1) | koff (s−1) | Kd (μM)a |
|---|---|---|---|
| 288 | 4.39 ± 0.18 | 375.8 ± 44.0 | 0.85 ± 0.11 |
| 293 | 5.26 ± 0.14 | 648.1 ± 72.2 | 1.23 ± 0.10 |
| 298 | 5.48 ± 0.20 | 933.5 ± 104.9 | 1.70 ± 0.48 |
| 303 | 7.03 ± 0.18 | 1641.9 ± 72.7 | 2.34 ± 0.41 |
| 308 | 7.42 ± 0.18 | 2519.3 ± 299.2 | 3.39 ± 0.47 |
| 313 | 9.79 ± 0.25 | 4233.2 ± 380 | 4.32 ± 1.03 |
a
The equilibrium dissociation constants were determined by detecting fluorescence intensity change of nSH3 domain upon binding to nSH3 domain (10).