Figure 1.

Schematic representation of the functionalization and coupling steps. The cleaned quartz glass surface (a) is first functionalized using APTES (b) to create amino groups. To these amino groups a PEG linker (c) is coupled with its activated carboxyl group and protected amino group. After deprotection of the PEG linker amino group, the surface can be functionalized in three ways, with only the lipid anchor bound to PEG (d), with only the protein anchor (protected trisNTA) bound to PEG (e), or with both anchors bound simultaneously to PEG (f). Afterward, a mixture of the protein with free lipid is applied to the surface, forming the final tBLM with reconstituted protein. To see this figure in color, go online.