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. 2016 Nov 1;111(9):1925–1934. doi: 10.1016/j.bpj.2016.08.051

Figure 2.

Figure 2

AFM scans (5 μm × 5 μm) in contact mode in buffer P1, with corresponding height profiles, represented as white horizontal lines in each image, below each scan. (a) The cleaned quartz glass surface shows a roughness of 511 PM. (b) Silanized quartz glass with APTES shows no change in surface roughness. (c) Binding PEG to silanized quartz reduces the roughness to 154.1 PM. (d) A surface functionalized only with the anchoring lipid SuccinylPE. (e) A surface functionalized only with the protein anchor trisNTA. (f) A surface functionalized with a mixture of 2:1 (mol/mol) SuccinylPE:trisNTA. To see this figure in color, go online.