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. 2016 Nov 7;7:13365. doi: 10.1038/ncomms13365

Figure 1. Biochemical assay demonstrating gephyrin is a SUMO substrate.

Figure 1

(a) In vitro SUMO reaction using purified proteins show that gephyrin is a substrate for SUMO-1 and SUMO-2 conjugation, higher migrating gephyrin SUMO bands were detected using Strep-tactin horseradish peroxidase (HRP). (b) In vivo detection of gephyrin SUMO1 and SUMO2 conjugation. Arrowheads: SUMO modified gephyrin bands. Higher exposure of the SUMOylated gephyrin bands is shown below. Lower panel shows loading controls and depletion of gephyrin after IP specifically in 3B11 lanes (c) FLAG–gephyrin interaction with myc–PIAS3 and myc–PIAS2α in HEK293 cells.