F420-dependent survival of M. smegmatis following antimicrobial challenge. The wild-type (WT), fgd mutant, and fbiC mutant strains were challenged with three antimicrobials (menadione, methoxsalen, and malachite green) during mid-exponential growth. The survival of the strains, as determined by CFU, is shown following administration of antimicrobials at 5 times the MIC of the wild-type strain. Error bars represent standard deviations from the results from three biological replicates. P values were calculated by comparing CFU counts between wild-type and mutant strains. ***, P < 0.001; ns, not significant.