FIG 5.

pKK22 was used to complement an hla mutant during a murine model of dermonecrosis. AH1263 (wild type [WT]) harboring pKK22 and hla mutant JB24 harboring pKK22 or pCK8 (pKK22 with hla) were used to inoculate SKH1 mice. (A) Representative infection site images (scale bar = 5 mm). (B) Calculated area under the curve (AUC) for area of dermonecrosis (ND, not detected). (C) Percent weight loss. (D) Bacterial burden at the site of infection. Data shown are median values plus 5th to 95th percentiles. All data were taken 3 days postinfection with 4 mice per group. ns, not significant by Mann-Whitney test for nonparametric data.