FIG 3.
Analysis of correlation between Pd-direct-qPCR and the reference plating or currently used PMA-direct-qPCR methods using 12-ml milk samples with 2.5-h enrichment. Counts obtained by the plating method, VRBA plate count; Pd-direct-qPCR, 50 μM Cl2(ŋ-cycloocta-1,5-diene)Pd(II)-qPCR without DNA extraction; PMA-direct-qPCR, 300 μM PMA-qPCR without DNA extraction. Twenty commercially pasteurized (live bacterium-free and anonymized) milk samples and 60 milk samples with live or dead E. coli artificially added were used.