TABLE 1.
Pd-direct-qPCR for discriminating between live and dead bacteriaa
| Agent concn (μM) |
CT |
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|---|---|---|---|---|---|---|---|---|---|---|
|
C. sakazakii (1.0 × 107 CFU or cells/ml) |
E. coli (1.0 × 107CFU or cells/ml) |
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| Dichloro(ŋ-cycloocta-1,5-diene) palladium(II) |
Bis(benzonitrile) dichloropalladium(II) |
Diamminedichloropalladium(II) |
Palladium(II) acetate |
Palladium(II) acetate |
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| Live | Dead | Live | Dead | Live | Dead | Live | Dead | Live | Dead | |
| 0 | 18.3 ± 0.78 | 19.8 ± 0.42 | 18.4 ± 0.59 | 18.1 ± 0.69 | 19.3 ± 0.63 | 19.0 ± 0.84 | 18.9 ± 0.61 | 19.9 ± 0.86 | 18.1 ± 0.29 | 20.4 ± 0.33 |
| 1 | 20.4 ± 0.21 | ND × 2 | 19.4 ± 0.13 | ND × 2 | ||||||
| 10 | 20.5 ± 0.88 | ND × 2 | 19.7 ± 0.97 | ND × 2 | ||||||
| 25 | 18.6 ± 0.35 | ND × 2 | ||||||||
a
C. sakazakii and E. coli cells were suspended in sterile distilled water. Dead cells were prepared by boiling in sterile distilled water for 3 min. qPCR (50 cycles) was performed in duplicate, and the CT values are presented as means ± SD (n = 2). ND × 2, no-amplification results were obtained in duplicate.