TABLE 3.

Accuracy, sensitivity, and specificity of milk data determined with Pd-direct-qPCR or PMA-direct-qPCR relative to the reference method of plating following EN ISO 16140:2003 validation^a

Method	Dairy matrix	PA	NA	ND	PD	Sum	% accuracyb	N+c	% sensitivityd	N−e	% specificityf
Pd-direct-qPCR	No-enrichment milk samples	26	44	10	0	80	87.5	36	72.2	44	100.0
	2.5-h-enrichment milk samples	36	44	0	0	80	100.0	36	100.0	44	100.0
PMA-direct-qPCR	No-enrichment milk samples	25	44	11	0	80	86.3	36	69.4	44	100.0
	2.5-h-enrichment milk samples	36	44	0	0	80	100.0	36	100.0	44	100.0

^aPA, number of samples showing positive agreement; PD, positive deviation; number of samples showing NA, negative agreement; ND, negative deviation; Sum, total numbers of samples.

^b% accuracy data were calculated as follows: 100 × (PA + NA)/Sum.

^cN⁺ data were calculated as follows: PA + ND.

^d% sensitivity data were calculated as follows: 100 × PA/N⁺.

^eN⁻ data were calculated as follows: NA + PD.

^f% specificity data were calculated as follows: 100 × NA/N⁻.