TABLE 4.

Accuracy, sensitivity, and specificity of milk data determined with Pd-direct-qPCR relative to the currently used PMA-direct-qPCR method in DNA elongation following EN ISO 16140:2003 validation^a

Dairy matrix	PA	NA	ND	PD	Sum	% accuracyb	N+c	% sensitivityd	N−e	% specificityf
No-enrichment milk samples	25	54	0	1	80	98.8	25	100.0	55	98.2
2.5-h-enrichment milk samples	36	44	0	0	80	100.0	36	100.0	44	100.0

^aPA, number of samples showing positive agreement; PD, positive deviation; number of samples showing NA, negative agreement; ND, negative deviation; Sum, total numbers of samples.

^b% accuracy data were calculated as follows: 100 × (PA + NA)/Sum.

^cN⁺ data were calculated as follows: PA + ND.

^d% sensitivity data were calculated as follows: 100 × PA/N⁺.

^eN⁻ data were calculated as follows: NA + PD.

^f% specificity data were calculated as follows: 100 × NA/N⁻.